pIRES-CD4t, a Dicistronic Expression Vector for MACS- or FACS-Based Selection of Transfected Cells
نویسندگان
چکیده
منابع مشابه
pIRES-CD4t, a dicistronic expression vector for MACS- or FACS-based selection of transfected cells.
To facilitate the selection of cell lines expressing transfected genes of interest, a plasmid vector has been constructed that directs the co-expression of heterologous cDNAs and a 3'-positioned cassette encoding a truncated CD4 marker. An encephalomyocarditis virus internal ribosomal entry site (IRES) mediates translational initiation from this 3' cassette, and a cytomegalovirus promoter drive...
متن کاملP-58: Which Novel Sperm Selection is Suitable for ICSI? MACS-DGC or DGC-MACS
Background: Super-paramagnetic annexin-V conjugated micro-beads are able to eliminate sperm with phosphatidylserine externalization (PSE). PSE is considered as apoptotic sperm. PES positive sperm can be removed from sperm preparations sample to enhance sperm quality for assisted medical procreation. Therefore, the efficiency of this technique over normal sperm processing procedure such as densi...
متن کاملA vector-based system for the differentiation of mouse embryonic stem cells toward germ-line cells
Objective(s):To culture thein vitro mouse embryonic stem cells (mESCs) and to direct their differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct Stra8-EGFP to select differentiated ES cells that entered meiosis. Retinoic acid was used to differentiate embryonic stem cells to germ cells. Materials and Methods: A fragment of Stra8 gene pr...
متن کاملHigh-level production of recombinant proteins in CHO cells using a dicistronic DHFR intron expression vector.
We have constructed expression vectors for Chinese hamster ovary (CHO) cells that produce both selectable marker and recombinant cDNA from a single primary transcript via differential splicing. These vectors produce stable CHO cell clones that, when pooled, produce abundant amounts of secreted recombinant proteins compared with the amounts produced by conventional expression approaches that hav...
متن کاملQuantitative Phosphorylation in (FACS) Purified CD4T+Human Thymocytes as a Function of IGF-1 Stimulation
PTK7, a catalytically inactive RTK, is a novel marker for human MHC-classII-restricted CD4+ RTEs and highly expressed during human and murine intrathymic development. It is implicated in neuroepithelial development, planar cell polarity and overexpressed in several cancers. Although surface PTK7 is useful in defining the phenotype of viable human CD4+ RTEs, its function in human T-cell developm...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99264st04